PaNonOLhe molecule e pāʻani ana i kahi hana koʻikoʻi ma nā hopena kaulike nui a hoʻohanaʻia ma kahi ākea o nā noi. No laila, he mea pono ia e loaʻa i kahi ala kūpono eʻike ai i ka phenol i nā'āpana likeʻole. Ma kēiaʻatikala, eʻimi mākou i nā hana'ē aʻe i loaʻa eʻike i ka phenol, ko lākou pono a me nā hana kūpono.

1.ʻO ka christography chrisotography (GC)

ʻO ka chromatographyʻo Chrisatography kahi mea i hoʻohana nuiʻia no kaʻikeʻana i ka phenol. Ma kēiaʻano, ua hoʻohālikelikeʻia kaʻatikala i loko o kahi kolamu i piha i kahi pā hale. ʻO ke kelepona kelepona a laila e kahe ana ma o ka kolamu, e hoʻokaʻawale i nā mea pilikino o ka hoʻohālike. Hoʻokumuʻia ka hoʻokaʻawaleʻana i ka piliona pili o nā'āpana o nā'āpana ma nā kahua o nā kikowaena a me nā kelepona kelepona.

Loaʻa: GC keʻano ikaika loa, kiko'ī, a me ka wikiwiki. Hiki iā ia ke nānā i nā haʻahaʻa haʻahaʻa o ka phenol.

Loaʻa i nā hemahema: Ponoʻo GC i nā limahana i hoʻomaʻamaʻa nui a me nā mea kūʻai aku i nā mea kūʻai aku, e hana ana i kahi kūpono o ke kula.

2. Kani chromatography (LC)

ʻO ka wai chromatography e like me ka sania chromatography, akā ua paʻiʻia ka paila i loko o kahi kolamu ma kahi o ke kākoʻoʻana i ke kākoʻo paʻa. Ua hoʻohana pinepineʻiaʻo LC no ka hoʻokaʻawaleʻana i nā molecules nui, e like me nā protein a me nā peptides.

Loaʻa: LC i kahi kiʻekiʻe kiʻekiʻe o ka hoʻokaʻawaleʻana a hiki ke mālama i nā moleki nui.

Loaʻa i nā hemahema: LC aʻoi aku ka paʻakikī ma mua o GC a e pono ai i ka manawa hou e loaʻa ai nā hopena.

3. Spectroscopy

ʻO Spectrosscopy kahi ala hanaʻoleʻole e pili ana i ke anaʻana i kaʻaeʻana i ka absorption a iʻole ka emission o ka radiation a me nā molekula paha. Ma ka hihia o ka phenol, infrared spectroscopy a me nā mea i hoʻopaʻaʻia e ka nmcerari magnetic (nmr) i hoʻohana pinepineʻia. Hoʻokomoʻia ka Spectroscopy i ka absorption o ka radiation infrared ma nā molepula,ʻoiaiʻo nmr spectroscopy e hoʻopili ana i ka radiofreques radiation e ka nucle o nā atomfere.

Loaʻa:ʻO SpectrossCOPPY koʻikoʻi a hiki ke hāʻawi i nāʻike kiko'ī e pili ana i keʻano o nā molecules.

Loaʻa i nā hemahema: Pono pinepine ka Spectorscopy e pono i nā mea kūʻai aku a hiki ke lilo i manawa manawa.

4. Nā Kūlana kala

Hoʻokomoʻia nāʻano kala kala e pili ana i kahi hoʻohālike me kahi uku e hana hou ai i kahi huahana kala e hiki ke ana i ka scctroopitally. ʻO keʻano maʻamau o ke kala maʻamau no kaʻikeʻana i ka phenol e pili ana i ka hanaʻana i ka sample me 4-aminonipyrine i ke alo o kahi coupling. ʻO ka ikaika o ke kala e kūlike pololei i ka nānāʻana o ka phenol i ka hoʻohālike.

Loaʻa nā hana: He maʻalahi keʻano o nāʻano kala

Loaʻa i nā hemahema: E loaʻa paha nāʻano hoʻololi i nāʻano kiko'ī aʻaʻole hiki keʻike i nāʻano āpau o Phenol.

5.ʻO nā waiwai waiwai

ʻO ka loaʻaʻana o nā mea i loaʻa i nā hopena o nā mea lapaʻau eʻike ai i nā mea ola eʻike i keʻano, nā waiwai, a me nā manaʻo o nā mea i manaʻoʻia. ʻO ka laʻana, hiki i kekahi mau mea ke hoʻohuli i ka phenol i kahi huahana kala i hiki ke ana i ka scctrophotomally. ʻO kēia mau mea waiwai he mea nui loa akāʻaʻole paha e nele i kaʻike i nā haʻahaʻa haʻahaʻa.

Loaʻa nā pono: He kiko'ī nui loa nā waiwai waiwai: a hiki ke hoʻohanaʻia no kaʻikeʻana no kaʻikeʻana i nā mea hoʻopili novel.

Loaʻa i nā hemahema: E loaʻa paha nā waiwai waiwaiʻo Biology